Title: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe
Authors: Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J.
Journal: Clin Vaccine Immunol,14(3):293-8 (2007)
Number of citations (Google Scholar): 32
A single-platform volumetric flow cytometer, the Partec Cyflow SL_3, was evaluated against a BD FACSCalibur/
Sysmex XT1800i dual platform for measuring CD4 lymphocytes, total lymphocytes, and the percentage of CD4
lymphocytes in whole-blood samples for monitoring the immune systems of human immunodeficiency virus
(HIV)/AIDS patients. Statistical analyses for precision, correlation, and agreement were performed. Coefficients
of variation (CV) of 5.8, 4.6, and 3.9% were obtained for low, medium, and high CD4 cell counts,
respectively, using the SL_3, and CV of 3.7, 4.0, and 0.94 were obtained for the same categories, using the BD
FACSCalibur. Significant correlations (P < 0.005) between the two assays for CD4 counts, total lymphocyte
counts, and percentages of CD4 were obtained, with correlation coefficients of 0.99, 0.96, and 0.99, respectively
(n 229). Using the Bland-Altman plot, mean biases of 18 cell/l (95% confidence interval (CI); 91 to 54
cells/l), 0.8% (95% CI; 3.6 to 2%), and 36.8 cells/l (95% CI; 477 to 404 cells/l) were obtained for
comparisons of CD4 counts, percentages of CD4 cells, and total lymphocyte counts, respectively.
The effects of
the age of the samples on the three parameters were also analyzed by comparing results from the same samples
analyzed at 6, 24, and 48 h after collection. The correlation coefficients for comparisons among different time
points for the same machine and among all the time points for the two different machines were greater than
0.90. These data showed that the Partec Cyflow SL_3 assay is comparable to the BD FACSCalibur_Sysmex
XT1800i dual-platform method for measuring the amount of CD4 cells and total lymphocytes and the
percentages of CD4 cells in blood samples for the purpose of monitoring HIV/AIDS patients.
Citation: Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J. Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe Clin Vaccine Immunol,14(3):293-8 (2007).